Aim of the present study to evaluate the antimicrobial activity of methanolic extract from L. elongata collected from Rameswaram coast southeast coast of India. The sea urchin were extracted with methanol and concentrated by using rotary evaporator at 40°C. The methanolic extract was tested by agar well diffusion method against 5 human pathogenic bacteria, named as Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholera and Klebsiella oxytoca. The highest activity was observed for K. oxytoca and V. cholerae at 20 mm and 17 mm respectively; whereas the S. typhi and S. aureus showed the lowest activity at 14 mm and 13 mm at 1000 µg/ml respectively. The Minimal inhibitory concentration (MIC) was estimated, the broth dilution method that was observed as 400 µg/ml for K. oxytoca; whereas the V. cholerae observed at 800 µg/ml, which the S. typhi and K. pneumoniae reported MIC at 1000 µg/ml respectively. The methanolic extract of L. elongata showed good antibacterial activity against the clinical pathogens. The present findings on the sea urchin demonstrate that this species may form the basis for the source of active manner of antibacterial potential in future.
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